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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: Science. 2009 Oct 29;326(5958):1369–1373. doi: 10.1126/science.1178535

Fig. 4. Interaction of the PCC with the ribosome and the nascent chain.

Fig. 4

(A-C) Molecular models for rRNA and ribosomal proteins are shown as in Fig. 2 E, for the Ssh1 complex as in Fig 3. Views focus on the cytosolic half of the Ssh1 model (A) and the cytosolic loops L6, L8 and the C-terminus (B). (C) A close-up on interactions of cytosolic loops L6 and L8. The positions of the conserved R278 and R411 are indicated (green). (D) Purified Ssh1 complexes from wild type and L6 and L8 mutants were incubated in the presence or absence of yeast ribosomes before centrifugation yielding supernatant (S) and pellet (P) fractions. After SDS-PAGE, Sbh2p was detected using anti-FLAG antibodies.