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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: J Immunol. 2009 Nov 4;183(11):6989–6997. doi: 10.4049/jimmunol.0901386

Figure 11. siRNA against IRF3 blocks RV-induced IFN and ISG expression.

Figure 11

A. IRF3 siRNA or non-targeting siRNA was transfected into BEAS-2B while seeding. After transfection, cells were infected with RV1B, UV-irradiated RV1B (UV-RV1B) or sham. A. After infection, cell lysates were probed with anti-IRF3 antibody. (The blot shown is representative of 3 separate experiments.) B–H. Total RNA was extracted and the expression of IFN-β, IFN-λ1, IFN-λ2/3, IRF7, IP-10, IL-8 and GM-CSF was determined by qPCR. The expression of each target gene was normalized to GAPDH. Expression levels are represented as the fold increase vs. sham-infected, non-targeting siRNA-transfected cells. The y-axis has been broken in order to show the effects of siRNA on both basal and maximal gene expression. Bars represent mean ± SEM for four experiments; numbers on top of the bars indicate the fold increase compared to the sham-infected sample within its own siRNA group. (*p<0.05 vs. non-targeting siRNA-transfected RV1B-infected cells, one-way ANOVA.)