Figure 12. siRNA against TRIF, MDA5, but not RIG-I reduced RV1B-induced IRF3 dimerization.
A. RIG-I, MDA5, TRIF siRNA or non-targeting siRNA was transfected into BEAS-2B. After transfection, cells were infected with RV1B, UV-irradiated RV1B or sham. After infection, cell lysates were probed with anti-IRF3 antibody. Poly I:C served as a positive control. B. IRF3 dimer to monomer ratio was quantified by densitometry. (The blot shown is a representative of two experiments.)