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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: J Immunol. 2009 Nov 4;183(11):6989–6997. doi: 10.4049/jimmunol.0901386

Figure 4. RIG-I and MDA-5 siRNA knockdown efficiencies.

Figure 4

A, B. RIG-I or non-targeting siRNA was transfected into BEAS-2B cells. After transfection, cells were infected with RV1B, UV-irradiated RV1B (UV-RV1B) or sham. After infection, cell lysates were probed with antibodies against RIG-I (A) or MDA-5 (B). Note the inductions in RIG-I and MDA5 expression with intact RV, as well as the apparent degradation of MDA-5 following viral infection. C, D. MDA5 or non-targeting siRNA was transfected into BEAS-2B cells. After transfection, cells were infected with RV1B, UV-irradiated RV1B (UV-RV1B) or sham. After infection, cell lysates were probed with antibodies against either MDA5 (C) or RIG-I (D). (The blots shown are a representative of three separate experiments.)