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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: Cell Stem Cell. 2007 Jun 7;1(1):113–126. doi: 10.1016/j.stem.2007.03.002

Figure 7.

Figure 7

ATR deletion leads to bulge stem cell and follicle loss after depilation. (A) Bulge stem cells were detected by CD34 immunohistochemistry (green) in telogen phase (before depilation) and anagen phase (4 days after the 1st and 3rd depilations) follicles; nuclei were detected with DAPI (blue). Significant loss and delayed regeneration of follicle bulge stem cells were observed in ATRmKO skin following depilation, while the bulge regenerated normally in the control mice (arrows). All pictures were taken at 100× magnification. (B) Quantification of the data presented in (A) Two mice per group and 8 skin sections per mouse were used, and at least 12 follicles per section were analyzed. (C) Quantification of the average number of follicles per 1 mm of skin in control and ATRmKO mice before depilation and 4-6 weeks after each depilation. H&E stained skin sections were used. A significant decrease in follicle density in ATRmKO skin (n = 2-4 for each group) was observed. Error bars represent SEM. (*) p < 0.001 as calculated by Student's T-test.