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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Bioconjug Chem. 2009 Sep 14;20(10):1853–1859. doi: 10.1021/bc900217h

Figure 3.

Figure 3

Dose dependent uptake and specific targeting of binary dendrons determined via flow cytometry, with mean fluorescence plotted against the concentration of the dendron conjugate. Specific targeting of AF-G3(COOH)11.9(RGD)4.1 (4) was displayed in human umbilical vein endothelial cells (HUVEC) (A), and U87MG human glioblastoma cells (B). Blocking of 4 at all tested concentrations with a 200-fold molar excess of c(RGDyK) confirms specific targeting of the αVβ3 integrin by the dendron. A cell-line comparison between HUVEC, U87MG, and αVβ3 integrin deficient KB cells (C) shows dendron uptake is greater in cell lines with increased αvβ3 integrin expression, demonstrating the active targeting capability of the binary dendron. A significant increase in mean fluorescence was displayed for a co-dendron structure capable of carrying additional dye, (OH)24(AF)2G2-G3(COOH)11.9(RGD)4.1 (10), in HUVEC (D). The specific uptake of the co-dendron was confirmed by inhibition with free RGD as well as from a non-targeted control, azide-G2(AF)2(OH)24 (9). Error bars indicate standard deviation as computed from the half-peak coefficient of variation.