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. 2010 Aug 9;99(4):1110–1118. doi: 10.1016/j.bpj.2010.06.013

Figure 5.

Figure 5

Comparison of the polylysine-induced rundown of hERG and KCNE1-KCNQ1 currents. (A) Time course of hERG and KCNE1-KCNQ1 current rundown after 3 μg/mL polylysine application at time 0. hERG current was evaluated from the peak tail current of a 1-s step at +30 mV followed by repolarization at –100 mV repeated every 3 s (N = 8 cells). KCNE1-KCNQ1 current was evaluated from the current at the end of a 1.5-s step at +40 mV followed by repolarization at –60 mV repeated every 3.5 s (N = 11 cells). ∗p < 0.05; two-way ANOVA. (B) Deactivation time constants obtained from a monoexponential fit of the deactivating tail current before (ctrl) and after the polylysine-induced rundown. For KCNQ1, deactivation was measured when a minimal current persisted (hERG, N = 8 cells; KCNQ1, N = 11 cells). ∗∗p < 0.01; ∗∗∗p < 0.001.