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Principle of α determination by DPMD. Left: Microfiberoptic detection of the fluorescence of a noninteracting, aqueous-phase dye in the tissue slice and overlying solution. The illumination/detection volume is approximately conical, with most of the light collected from near the fiberoptic tip. Right: Schematic of DPMD data showing α determination from the ratio of the (background-subtracted) dye fluorescence in the slice to that in the overlying solution.
