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. 2010 Aug 9;99(4):1239–1246. doi: 10.1016/j.bpj.2010.06.015

Figure 1.

Figure 1

Soluble and aggregated forms of the two proteins display different antibody-binding characteristics during amyloidogenesis. (A) Sequence of HET-s(218-289) (WT) and M8 toxic mutant. (B) Detection of soluble proteins (pH 2.0 just after desalting) and amyloid fibers formed after several days at pH 7.4 with Ponceau Red and antihistidine antibody (α-His Ab). (C) Kinetics of polymerization followed with anti-His-tag antibody demonstrate that this antibody shows almost no binding affinity for soluble WT (T0 at pH 7.4) or M8 amyloid fibers. Dots represent 2-μL drops of 20 μM proteins grown without agitation at pH 7.4 and 37°C.