Table 1.
Transcription activity is sensitive to mechanical state of the template
| Species | Twist | Size (bp) | kfor (×10−3 s−1) | kdiss(stalled) (×10−3 s−1) | kcat (nt × s−1) | kdiss (×10−3 s−1) | Processivity (×103 nt) |
|---|---|---|---|---|---|---|---|
| +UT | − | 100 | 2.0 (1.3, 4.1) | 2.5 (1.8, 4.2) | 15.6 (12.6, 19.8) | 2.3 (2.0, 2.8) | 6.8 (6.4, 7.2) |
| UT | − | 108 | 4.8 (3.4, 8.0) | 3.1 (2.3, 4.9) | 4.1 (3.2, 5.4) | 0.6 (0.5, 0.8) | 6.7 (6.2, 7.2) |
| OT | + | 106 | 2.4 (1.8, 3.6) | 3.6 (2.8, 5.0) | 4.2 (2.4, 8.4) | 0.3 (0.2, 0.5) | 14.4 (11.2, 17.7) |
| Control | − | 2536 | ∼1.3 | ∼5.1 | ∼130 | n/a∗ | >625 |
Mean values for kfor, kdiss(stalled), kcat, kdiss, and processivity are determined from the best-fit exponential function fitted to data. The 95% confidence intervals from the fits are shown in parentheses.
∗
These data were best fit to a linear function and therefore are not well described by an exponential constant.