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. 2010 Aug 9;99(4):1303–1310. doi: 10.1016/j.bpj.2010.06.005

Figure 2.

Figure 2

uPAINT imaging with two different ligand/receptor systems. (A) Wide-field fluorescence image of a fibroblast expressing TM-6His and GFP. (B) Superresolved image of TM-6His labeled with trisNTA-AT647N obtained by uPAINT and (C) corresponding trajectories. (D–F) Same as panels A–C, but for GPI-GFP labeled with anti-GFP-AT647Ns on a COS 7 cell. (G) Surface density of the trajectories recorded on TM-6His/GFP or GPI-GFP transfected cells (19,191 and 31,936 trajectories, respectively), and on GFP-transfected control cells for the two protein/ligand complexes (n = 4–5 cells in each condition). (H) Distributions of the trajectory lengths measured with trisNTA-AT647N and anti-GFP-ATTO-647Ns. (Solid line) Fit with an exponential curve. (I) Cumulative number of trajectories as a function of time for the two protein-ligand complexes.