Figure 3.

Statistical analysis of GPI-GFP spatio-temporal localization on COS 7 cells. (A) Distribution of instantaneous diffusion constants for GPI-GFP on COS 7 cells. Control corresponds to cells expressing GFP alone. (B) Four microdomains presenting a high surface density of GPI-GFP are highlighted on a superresolved image. (C) Corresponding color-coded two-dimensional map of the mean GPI-GFP step lengths during 50 ms within 200 × 200 nm² pixels. (D) Two-dimensional plot of the molecule densities as a function of mean step lengths measured within 200 × 200 nm² pixels (n = 487,112 steps, five cells) revealing reduced movements in the high density regions.