Figure 2.

Specificity of E-selectin-mediated adhesion. (A) The frequencies of flowing neutrophils tethering to a chamber floor coated with BSA or 5 μm⁻² E-selectin in the absence or presence of 20 μg/mL of an anti-E-selectin blocking antibody (ES1) or 5 mM EDTA measured in a range of wall shear stresses. Representative data of five or more experiments. (B) The binding frequencies of E-selectin-coated beads to beads bearing streptavidin or sLe^x in the absence or presence of ES1. Data are presented as the mean ± SE of three pairs of beads.