Table 1.
Mono- and Dimethylated Amino Acid Residues Identified in p53, SMC3, MeCP2, and iNOs
| protein | peptide sequence | methylated AA residue |
|---|---|---|
| p53(gi:506435) | 7 DPSVEPPLSQETFS20 | E11/E17 (+14 Da)a |
| 49DIEQWFTEDPGP60 | E51 (+14 Da) | |
| 102TYQGSYGFR110 | R110 (+14 Da) | |
| 111LGFLHSGTAK120 | H115 (+14 Da) | |
| 203VEYLDDR209 | E204/R209 (+14 Da)a | |
| 208DRNTFRHSVVVPYEPPEVGS227 | R209/R213/E221/E224 (+14 Da)a | |
| 293GEPHHELPPGSTK305 | E295 (+14 Da) | |
| 322PLDGEYFTLQIR333 | E326 (+14 Da) | |
| SMC3 (gi:4885399) | 62LALLHEGTGPR72 | R72 (+14 Da) |
| 144INQMATAPDSQR155 | R155 (+14 Da) | |
| 222ALEYTIYNQELNETR236 | R236 (+14 Da) | |
| 239LDELSAK245 | K245 (+14 Da) | |
| 265DKMEDIER272 | R272 (+14 Da) | |
| 311DLQDELAGNSEQR323 | R323 (+14 Da) | |
| 401SLDQAINDK409 | K409 (+14 Da) | |
| 419DLEDTEANKEK429 | K427/K429 (+14 Da)a | |
| 437LDQDLNEVK445 | K445 (+14 Da) | |
| 475EENAEQQALAAK486 | K486 (+14 Da) | |
| 635SMEVSTQLAR644 | K644 (+14 Da) | |
| 986ALDQFVNFSEQK997 | K997(+14 Da) | |
| MeCP2(gi:4826830) | 257AEADPQAIPK266 | E258 (+14 Da) |
| 295SVQETVLPIK304 | E298 (+14 Da) | |
| 436TQPAVATAATAAEK449 | E448 (+14 Da) | |
| 201AATSEGVQVKR211 | K210 (+28 Da) | |
| 116SAGKYDVYLINPQGK130 | K119 (+28 Da) | |
| iNOS (gi:6754872) | 408AVTEINVAVLHSFQK422 | K422 (+14 Da) |
| 1031VLFQVHTGYSR1041 | R1041 (+14 Da) | |
| 1105LIQSPPEPLDLNR1118 | E1110/R 1118 (+14 Da)a |
a
Either residue was methylated individually, but no dimethylation was ever observed on the same peptide.