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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Nature. 2010 Jun 6;465(7300):942–946. doi: 10.1038/nature09076

Figure 3.

Figure 3

mTOR reactivation inhibits autophagy and initiates lysosome reformation. (a) TEM (bottom, scale bar = 5 um) or fluorescence (top, scale bar, 10 um) of NRK-LC3 cells starved for hours (h) with enlargements (boxes). (b) and (d) immunoblots of (a) with indicated antibodies after transfection (d) with non-specific (NS) or atg5 RNAi. (c) Autophagy (black lines = independent experiments) and phospho-S6K (densitometry of b, red line). (e)-(g) Two hour starved LAMP1-YFP-NRK-LC3 cells treated with 100 nM rapamycin for another 4 (6 h) or 6 (8 h) hours analyzed by blotting (e) as in (b) or microscopy (f, scale bar, 5 um) with quantitation (g). Error bars show s.e.m (n=3).

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