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. 2010 Jun 29;17(4):223–231. doi: 10.1093/dnares/dsq015

Table 1.

List of primers derived from E. gracilis mRNA sequences

mRNAa Referenceb Accession numberc Forward primersd Reverse primerse
Eno29 51 AJ272112 65–84 298–279
GapA 26 L21904 23–42 468–449
Pbgd 52 X15743 183–202 511–492
PetA 53 AF443625 49–68 422–403
PetJ 19 AJ130725 89–108 386–367
PsbO 54 D14702 40–59 674–655

aPrimers were derived from mRNA sequences of nucleus-encoded genes (Eno29, GapA, Pbgd, PetA, PetJ and PsbO) for chloroplast proteins (enolase, glyceraldehyde-3-phosphate dehydrogenase, porphobilinogen deaminase, cytochrome f, cytochrome c6, and 30 kDa protein of the oxygen-evolving complex, respectively).

bNumber of reference in the reference list in which the corresponding mRNA was characterized.

cAccesion numbers of mRNAs.

d,eThe numbers of primers correspond to the positions in mRNA sequences that can be found under the accession numbers (accession number) listed in the third column. For example, forward primer 65–84 (first row, fourth column) is identical to positions 65–84 of Eno29 mRNA sequence, which can be found under accession number AJ272112 and reverse primers 298–279 (first row, fifth column) is complementary to the sequence 279–298 of Eno29 mRNA.