Table 2.

List of primers derived from E. gracilis EST sequences

EST producta	Accession numberb	Organism with the best BLASTX hit	E-value	Forward primersc	Reverse primersd
PetF	EG565162	Euglena viridis	100E−40	57–76	627–608
PsaF	EG565174	Chlamydomonas reinhardtii	900E−36	30–49	499–480
PsbM	EG565161	Ostreococcus tauri	700E−11	85–104	575–556
PsbW	EG565140	Bigellowiella natans	300E−14	68–86	560–543

^aThe name of protein product of ESTs from which primers were derived. PetF is plastid-targeted ferredoxin, PsaF is subunit F of photosystem I, and PsbM and PsbW are subunits M and W of photosystem II.

^bAccession numbers of ESTs.

^c,dThe numbers of primers correspond to the positions in corresponding ESTs. E.g. forward primer 57–76 (first row, fifth column) is identical to positions 57–76 of EST with accession number EG56162, and reverse primer 627–608 (first row, sixth column) is complementary to nucleotides 627–608 of this EST.