Table 4.
Primary sequence identity (top-right half) and alignment P-value (bottom-left half) of the selected introns from E. gracilis (44–50 bp long, with consensus GT/AG borders)
| eno29-i1 | gapA-i1 | pbgd-i1 | pbgd-i2 | pbgd-i3 | petA-i1 | petF-i1 | psaF-i1 | psbM-i1 | psbW-i1 | nop1p-i1 | nop1p-i3 (%) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| eno29-i1 | 45% | 60% | 27% | 42% | 36% | 61% | 56% | 55% | 47% | 55% | 42% | |
| gapA-i1 | 0.07 | 46% | 37% | 52% | 49% | 39% | 54% | 55% | 45% | 44% | 37% | |
| pbgd-i1 | 0.01 | 0.39 | 55% | 40% | 43% | 51% | 53% | 49% | 42% | 46% | 32% | |
| pbgd-i2 | 0.32 | 0.35 | 0.20 | 55% | 33% | 53% | 46% | 29% | 47% | 41% | 41% | |
| pbgd-i3 | 0.22 | 0.03 | 0.93 | 0.23 | 42% | 47% | 50% | 37% | 53% | 37% | 47% | |
| petA-i1 | 0.20 | 0.02 | 0.25 | 0.27 | 0.25 | 38% | 56% | 34% | 46% | 49% | 38% | |
| petF-i1 | 0.01 | 0.21 | 0.15 | 0.02 | 0.90 | 0.63 | 44% | 55% | 46% | 48% | 47% | |
| psaF-i1 | 0.33 | 0.15 | 0.05 | 0.74 | 0.13 | 0.03 | 0.05 | 38% | 40% | 46% | 39% | |
| psbM-i1 | 0.03 | 0.09 | 0.16 | 0.83 | 0.37 | 0.28 | 0.11 | 0.91 | 47% | 50% | 39% | |
| psbW-i1 | 0.01 | 0.06 | 0.20 | 0.34 | 0.05 | 0.24 | 0.26 | 0.07 | 0.27 | 50% | 46% | |
| nop1p-i1 | 0.04 | 0.00 | 0.26 | 0.44 | 0.83 | 0.28 | 0.36 | 0.02 | 0.01 | 0.35 | 58% | |
| nop1p-i3 | 0.38 | 0.31 | 0.34 | 0.95 | 0.58 | 0.65 | 0.23 | 0.62 | 0.25 | 0.93 | 0.04 |
Except for nop1p-i1 and nop1p-i3 (introns present in the gene-encoding nucleolar protein fibrillarin), all these introns are present either in the presequence-encoding regions or shortly upstream of them. The primary sequence identity was calculated as the number of identical nucleotide oppositions of two introns in a pairwise alignment divided by the length of the alignment. Statistically significant alignments with P-value ≤0.05 are shown in bold (see section Methods).