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. 2010 Jun 29;17(4):245–260. doi: 10.1093/dnares/dsq016

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© The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Promoter levels of RNA polymerase II and histone modifications. HeLa resting (0 h) and serum-treated cells (1, 6, and 24 h) were used in ChIP assays with antibodies against RNAPII, H3, H3Ac(K9/K18), H3K4me3, and H3K27me3. Isolated DNA was used in real-time PCR with primers to the promoters of HBB, HNRNPK, TUBB6, GAPDH, HNRNPH, RPS7, HSP70, and EGR1. Data are expressed as percent of input chromatin for RNAPII, or as ratios of modified histone marks to total H3 ChIP signals. Data represent the mean ± sd of four independent experiments.