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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):5172–5178. doi: 10.4049/jimmunol.0903759

Figure 1.

Figure 1

NK cells produce IFNγ in vitro in response to co-culture with Lm-infected BMDCs. (A) CD11c+CD11b+ BMDC were derived from BM cells cultured in GM-CSF. Spleen cells were processed via nylon wool columns to enrich for lymphocytes. These nylon wool non-adherent (NWNA) cells were co-cultured with wt Lm or Δhly Lm-infected BMDC for 19hrs. (B and C) NK cells produced IFNγ only in response to wt Lm infection as measured by intracellular staining and ELISA. (D) Kinetics of IFNγ production by NWNA responding to wt Lm in infected BMDC as measured by ELISA.