Skip to main content
. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):5172–5178. doi: 10.4049/jimmunol.0903759

Figure 5.

Figure 5

IL-18 is necessary IFNγ production by naïve NK cells. (A) wt Lm-infected BMDC co-cultures were treated with the caspase-1 specific inhibitor Z-WEHD-FMK at 100μM. Caspase-1 inhibition abrogated IFNγ induction. Addition of 500 pg/ml exogenous IL-18 restored IFNγ induction, but 2.5 ng/ml of exogenous IL-1β did not. Specific inhibition of IL-18 (1μg/ml of αIL-18) significantly reduced IFNγ induction in co-cultures infected with wt Lm. (B) Addition of recombinant IL-18 to Δhly Lm infected co-cultures restored IFNγ induction to levels seen with wt Lm infection. (C) wt Lm induction of IFNγ requires MyD88 expression on NWNA. C57BL/6 and MyD88−/−BMDC were infected with wt Lm and co-cultured with either C57BL/6 or MyD88−/−NWNA. Only co-cultures with MyD88−/− NWNA failed to induce IFNγ in response to Lm infection.