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. 2010 Apr 26;87(4):723–731. doi: 10.1093/cvr/cvq117

Figure 6.

Figure 6

Inhibition of tube formation on reconstituted basement membrane by MEOX2 requires the presence of both the N-terminal domain and the homeodomain. (A) Tube formation. HUVECs were transfected with pcDNA3.1-MEOX2 and the four MEOX2 deletion constructs as described and then plated on reconstituted basement membrane in Materials and Methods. Cells were incubated overnight and then photographed. (B) Quantification of tube formation. Tube formation was quantified as described 9,10,22 and the mean ± standard deviation for tubes/low powered field (LPF) were calculated. In spite of a transfection efficiency of only 30% (not shown), MEOX2 and MEOX2ΔCT reduced tube formation by nearly 50% (P < 0.01).