Figure 1. Production of HCP from EHEC EDL933 strain.

Flow cytometry data from the EHEC EDL933 strain cultured in different media showing optimal production of HCP after bacterial growth in Minca (A). Western blot confirming HCP production by EDL933 in Minca (B). The EDL933ΔhcpA strain was used as a negative control (C). Detection of DnaK with anti-DnaK antibody was used to ensure that equal amounts of antigen were tested.