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. 2010 Aug 12;5(8):e12127. doi: 10.1371/journal.pone.0012127

Figure 4. HCP and HcpA selectively activate release of IL-8 and TNF-α.

Figure 4

HCP and HcpA induced release of IL-8 and TNF-α but not IL-2, IL-6, and IL-10 (A). *P<0.0001 and HcpA **P<0.001. High levels of IL-8 and TNF-α released from HT-29, T84, and HeLa cells in the presence of HCP and HcpA (B) and (C). HCP and HcpA induced similar levels of IL-8 (1658.3 pg/ml from HT-29; 1558.33 pg/ml from T84; and 1423 pg/ml from HeLa cells) (C). Both proteins induced comparable levels of TNF-α release from HT-29, T84, and HeLa cells [HCP (1346.6, 1258.3, and 1205.3 pg/ml) and HcpA (1272.6, 1205.3, and 1223.3 pg/ml)], respectively (B). Polarized intestinal and non-intestinal cells were incubated alone with DMEM as a negative control.