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Purified HCP or HcpA protein (100 pg/ml) were pre-incubated with anti-HCP antibodies in DMEM low glucose medium before addition to the apical surface of HT-29 cells. A dose-dependent inhibition for IL-8 and TNF-α from HT-29 cells was obtained: between 95–98% at the 1∶200 dilution of the antibody (A and B). *P<0.0002.
