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. 2010 Aug 12;5(8):e12120. doi: 10.1371/journal.pone.0012120

Figure 1. Enrichment in pp65495–503/A*0201- or BZLF154–64/B*3501-specific T cells after sorting of CD8 T cells with pMHC magnetic multimers.

Figure 1

(A) CD8 T cells, derived from the PBL from D01 and D03 were sorted with 245V mutated pp65495–503/A*0201 multimers, then expanded in culture, and stained with PE-conjugated pp65495–503/A*0201 tetramers and FITC-conjugated anti-CD3. (B) CD8 T cells derived from the PBL from D15 were sorted with 245V mutated BZLF154–64/B*3501 multimers, and then expanded in culture. Unsorted and sorted T cells were stained with PE-conjugated BZLF1/B35 tetramers and FITC-conjugated anti-CD3. The percentage of positive cells is indicated in the upper right quadrant.