Table 1. Screening of CD8 T cell lines enriched in HCMV- or EBV-specific T cells for cross-reactivity to allogeneic MHC molecules.
Donor# | Molecular HLA class I typing | % of tetramer-positive T cells | Reactivity to allogeneic HLAa | |||||
HLA-A* | HLA-B* | HLA-Cw* | ||||||
pp65/A*0201-sorted T cell lines | ||||||||
D01 | 0201 | 0301 | 3501 | 4402 | 0401 | 0501 | 97 | A*3101 |
D02 | 0201 | 33 | 08 | 14 | 07 | 08 | 98 | none |
D03 | 0201 | 0201 | 1302 | 5101 | 0202 | 0602 | 99 | A*3201 |
D04 | 0201 | ND | ND | ND | 98 | none | ||
D05 | 0201 | ND | ND | ND | 99 | none | ||
D06 | 0201 | ND | ND | ND | 99 | none | ||
D07 | 0201 | 2301 | 2705 | 4402 | 0102 | 0509 | 98 | none |
D08 | 0201 | 2301 | 4101 | 4402 | 0501 | 1701 | 89 | A*3001 |
D09 | 0201 | 30 | 08 | 44 | 04 | 07 | 98 | none |
D10 | 0201 | 0201 | 1501 | 4403 | 0304 | 0501 | 95 | none |
D11 | 0201 | 1101 | 0801 | 2705 | 01 | 07 | 97 | none |
BMLF1/A*0201-sorted T cell lines | ||||||||
D12 | 0201 | 3201 | 15 | 15 | 0303 | 04 | 93 | none |
D13 | 0201 | 0201 | 2705 | 4002 | 0102 | 1501 | 95 | none |
BZLF1/B*3501-sorted T cell lines | ||||||||
D14 | 0301 | 1101 | 0701 | 3501 | 04 | 07 | 92 | none |
D15 | 2402 | 3101 | 3501 | 4001 | 03 | 07 | 97 | Cw*0602 |
D16 | 2402 | 3201 | 2705 | 3501 | 01 | 04 | 97 | none |
CD8 T cell lines were screened on COS-7 cells transfected with individual HLA-encoding cDNA and TNF-α production was measured after a 6h-coculture. All T cell lines were PBL-derived. ND : not determined.