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. 2010 Aug 12;5(8):e12119. doi: 10.1371/journal.pone.0012119

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Briatore et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A1,A2) Double labelling for GABA_ARγ2 (green) and NL2 (red) in control (A1) and PC-Δα1 mice (A2). NL2 colocalizes extensively with the γ2 subunit and also clusters at postsynaptic sites lacking GABA_ARs in Purkinje cells of PC-Δα1 mice. (B,C) Electron micrographs of the ML of a PC-Δα1 mouse showing that GABA-immunopositive axon terminals (Ax) make both conventional, symmetric synapses (B, arrowheads) with Purkinje cell dendrites (Pc) and heterologous synapses (C) with spines (Sp). (D1,D2) Similar distribution of α3-GABA_ARs in the ML of control (D1) and PC-Δα1 mice (D2). PCL, Purkinje cell layer. Scale bars: A = 15 µm. B,C = 200 nm. D = 20 µm.