Table 1. Observed and deduced parameters of wild type and mutant cells.

Property	Symbol	Units	WT 1h	WT 3h	WT 5h	WT 7h	gc-null	pla2-null	sgc/pla2-null	ddia2-null
Observed pseudopod (n cells/pseudopodia)			7/215	8/256	28/835	7/294	7/312	8/208	8/219	8/164
Pseudopod size	λ p	µm	5.0±0.2	5.3±0.2	5.2±0.2	4.7±0.2	4.6±0.4	7.7±0.5	5.3±0.7	5.6±0.4
Splitting angle	ϕ	degrees	62	58	55	55	54	50	54	54
SD splitting angle	σϕ	degrees	26.1	29.7	27.8	27.5	26.9	28.5	27.5	46.5
Alternating	a	-	0.74±0.02	0.74±0.06	0.77±0.04	0.82±0.06	0.67±0.05	0.68±0.05	0.75±0.08	0.75±0.03
Fraction splitting	s	-	0.55±0.07	0.60±0.05	0.86±0.06	0.89±0.05	0.71±0.06	0.67±0.10	0.41±0.07	0.82±0.09
Observed dispersion (20 cells)
Correlation factor	γobs	-	0.46±0.11	0.52±0.07	0.74±0.09	0.81±0.10	0.58±0.11	0.55±0.11	0.35±0.08	0.53±0.07
Turn angle	θ	degrees	63	59	42	36	55	57	70	58
Calculated dispersion
Monte Carlo correlation factor	γMC	-	0.40	0.46	0.65	0.70	0.53	0.51	0.35	0.46
Equation (9) correlation factor	γstep	-	0.40	0.42	0.64	0.69	0.50	0.48	0.31	0.43

The observed pseudopod data were derived from wild type (WT) cells starved for the indicated times, or from mutants starved for 5h. Indicated are the number of cells and pseudopodia analyzed. The data for λ _p are the means and SEM for pseudopodia; the data for ϕ and σ_ϕ are the means and SD for pseudopodia; the data for a and s are the means and SEM for cells. The data for the observed γ_obs were obtained by least square curve fitting to Eq. 4 of the dispersion of 20 cells during 15 minutes; the error indicates the 95% confidence level of the fit; θ = cos⁻¹ γ. The data for the MC simulated γ_MC were obtained by simulating 100,000 trajectories using the observed pseudopod parameters, and the corresponding value of γ was computed as described for the experimentally observed γ. Finally, γ_step was calculated with Eq. 9, only using pseudopod parameters.