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. 2010 Aug 12;6(8):e1000880. doi: 10.1371/journal.pcbi.1000880

Figure 4. The results of CDC25c peptide and CDC25c phosphopeptide binding to the PBD by ITC.

Figure 4

ITC data for the binding of CDC25c peptide and CDC25c phosphopeptide to the PBD of PLK1. The ITC traces and the determined thermodynamic parameters of binding are shown. The CDC25c peptide displays no detectable binding. The binding of the CDC25c phosphopeptide can be fit to a Kd of 0.705 µM. Errors in thermodynamic parameters are derived from the fitting error after repeating the experiment at least three times.