Figure 6. Transfer of CD11b⁺Ly6C⁺ TIP-DC precursors increases pathogenicity during T. brucei infection.

CD11b⁺Ly6C⁺ monocytic cells were isolated from the bone marrow of WT or TNF KO mice on day 6 of T. brucei infection, labeled with CellVue and transferred intravenously into infected recipient CCR2 KO mice. Twenty four hours after transfer blood serum was analyzed for TNF concentration and ALT activity. Data are mean ± SEM of three individual mice of one of three independent experiments performed. Note that due to the low numbers of transferred cells recovered from the liver, we were unable to assess iNOS or TNF expression on transferred cells using intracellular staining assays. *, significantly higher (p<0.05) compared to CCR2 KO mice that did not receive CD11b⁺Ly6C⁺ monocytic cells.