Figure 5. Binding of _FLAGlysin_SM1 to immobilized fibrinogen fragments D and E.

(A) Schematic diagram of human fibrinogen. The Aα, Bβ, and γ chains, major disulfide linkages, and plasmin cleavage sites are shown. (B) Indicated concentrations of _FLAGlysin_SM1 were incubated with immobilized human fibrinogen, fibrinogen fragment D, or fragment E. Bound _FLAGlysin_SM1 was detected with anti-FLAG antibody. Bars indicate the means (± S.D.). (C) Fibrinogen fragment D was separated by SDS-PAGE under reducing conditions and stained with Coomassie blue (panel 1) or transferred onto nitrocellulose membrane and incubated with _FLAGlysin_SM1 (5 µg/ml). The bound proteins were detected with anti-FLAG antibody (Lane 2). Arrows indicate positions of Aα, Bβ, and γ chain fragments. Numbers indicate molecular mass (kDa).