Table 1.
Biotinylated lectin | Glycan structure detected |
---|---|
ConA | α-Linked mannose |
MAL | Sialic acid in an (α-2,3) linkage |
AAL | Fucose linked (α-1,6) to N-acetylglucosamine or to Fucose linked (α-1,3) to N-acetyllactosamine |
SNA | Sialic acid attached to terminal galactose in (α-2,6), and to a lesser degree, (α-2,3), linkage |
PNA | Galactosyl (β-1,3) N-acetylgalactosamine |
AAL aleuria aurantia, ConA, concanavalin A, MAL maackia amurensis II, PNA peanut agglutinin, SNA sambucus nigra (elderberry) bark.
(Reprinted with permission from Patwa TH, Zhao J, Anderson MA, Simeone DM, Lubman DM: Screening of glycosylation patterns in serum using natural glycoprotein microarrays and multi-lectin fluorescence detection. Anal Chem (2006) 78(18):6411-6421–2007 American Chemical Society) [19•].