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The osteoblast cell line, MC3T3 (A), the adipocyte cell line, 3T3-L1, (B) and 2 RA fibroblast-like synoviocyte (FLS) cell lines (C, D) were cultured for 3 days in the presence or absence of TGF-β, IL-1β, TNF-α, or IL-6 and supernatants were assayed for FSTL-1. Each bar represents the mean and S.E.M. of 6 replicates. *p < 0.05.
