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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arthritis Rheum. 2010 Aug;62(8):2370–2381. doi: 10.1002/art.27512

Figure 1. Stable knock-down (KD) of MMP-13 expression by retroviral transduction.

Figure 1

A. Real time PCR verification of MMP-13 KD by shOligos targeted to different MMP-13 exons. The loss of MMP-13 expression (mean percentage KD±SEM) were as follows: OE7 (3 patients) 91%±3.8; OE16 (6 patients): 83.1%±10,5. B. One representative experiment out of three different patients’chondrocytes tested in triplicate wells, where KD efficiencies were quantified by MMP-13 ELISA assay of cell supernatants in unstimulated or stimulated conditions with 100 nM chemokines (BCA, GROα, SDF) or 100 units/ml IL-1β for 72 hours. C. 3 week micromasses were left unstimulated or stimulated with 100 nM chemokines (BCA, SDF, Larc, GROα) or 100 units/ml IL-1β for 72 hours. MMP-13 KD statistically decreased MMP-13 release from IL-1 stimulated OE16 micromasses (p=0.046). Results were obtained from duplicate micromass cultures of 6 different patients.