Phylogenetic relationships between published NITR V domains. Published NITR sequences described from Southern pufferfish (S. nephelus, Sn), zebrafish (D. rerio, Dr), rainbow trout (Oncorhynchus mykiss, Om), channel catfish (I. punctatus, Ip), Japanese flounder (Paralichthys olivaceus, Po), medaka (O. latipes, Ol), T. nigroviridis (Tn) and European sea bass (D. labrax, Dl) were included in the alignment. Sequences are defined by the two-letter species abbreviation followed by the NITR gene number in that species. Only one NITR gene family member is incorporated; the other family members are indicated in parenthesis. NITR genes which encode a V and I domain in a single exon, V and I domain in two separate exons or only the V domain are highlighted with different colours. Sea bass NITR gene groups are also indicated. Bootstrap values are assigned to each interior branch; bootstrap values less than 50 are not shown. Branch lengths correspond to the number of amino acid substitutions, with the scale indicated at the bottom of the figure