Table 3.
Real-time PCR results normalized to β-actin expression levels
| Accession no. | Amplified RNA | PCR primer pair | Real- time PCR ΔCP normalized to β-actin expression | M-value | Competence between PCR ΔCPs and M-value |
|---|---|---|---|---|---|
| DQ850666 | Metallothionein | F: AAGGGAAGACCTGCGACACTAG | 4.5 | 2.04 | + |
| R: TCACAAGGGCAGCAAGCA | |||||
| DQ850591 | Carboxypeptidase B | F: CTGCTCTGCGTAGTCTGTACGG | −2.1 | −2.09 | + |
| R: GGAATACTTCACACCCAGGTCG | |||||
| DQ850208 | Carboxyl ester lipase | F: CAGAAGATGAGGCTGCGTTACG | −2.2 | −1.51 | + |
| R: GAAGTACAGGTGATGGCACAAGC | |||||
| DQ849772 | Complement component C3 | F:TTACTTGGCCCTCTTGTGTGG | 0.9 | −1.14 | − |
| R: CAACAGGCAGTCAAATCAAGCC | |||||
| DQ849874 | Cytochrome P4502k5 | F: TGGTTGAAGCTCATCAGAATTCC | 0.6 | −1.3 | − |
| R: CCCTTGCATGGATTCAGATAGG | |||||
| DQ850737 | β-actin | F: CATCCTGCGTCTGGACTTGG | |||
| R: GATTTCCCTCTCGGCTGTGG |
M-values were obtained from the microarray analysis.