Figure 3.

HIPK2 activates CREB-dependent transcription in a kinase-dependent manner. (A) CREB, 0.1 μg, along with 0.1 μg (+) or 0.3 μg (++) HIPK2-WT or HIPK2-KD plasmids were cotransfected with pCRE 4 copies (CRE4)-luciferase or pTATA-luciferase reporter into K562 cells. (B) CREB, 0.1 μg, along with 0.3 μg HIPK2-WT or HIPK2kd plasmids were cotransfected with rat BDNF promoter III-luciferase into K562 cells. In both A and B, cells were harvested after 24 h to measure luciferase expression. Relative luciferase activity is shown by setting each reporter transfection alone as 1.0. Average of four experiments are shown with SEs.