Table 2.
Data Collection and Refinement Statistics
| Data Collection | murine Igβ monomer |
murine Igβ homodimer |
human Igβ homodimer |
|---|---|---|---|
| Space group | P21212 | P41212 | P4132 |
| Unit cell (Å) | a=35.63, b=79.72, c=34.32 | a=b=87.97, c=75.29 | a=129.83 |
| Resolution limit (Å) | 1.7 | 3.1 | 3.2 |
| Unique reflections | 10471(898)a | 5597(541) | 6616(630) |
| Redundancy | 5.5(2.3) | 6.1(5.4) | 27.6(28.7) |
| Completeness (%) | 93.8(67.4) | 97.4(98.7) | 99.9(100.0) |
| Rsym (%)b | 6.5(31.7) | 13.5(50.6) | 7.0(33.9) |
| <I/σ(I)> | 21.5(2.6) | 14.1(3.2) | 55.6(13.1) |
|
| |||
| Refinement | |||
| Resolution (Å) | 40.-1.7 | 48.-3.1 | 46.-3.2 |
| No. reflections | 10071 | 5407 | 6407 |
| No. protein atoms | 757 | 1485 | 1678 |
| No. solvent atoms | 92 | 26 | 2 |
| Rcryst(%) | 18.7(27.0) | 20.6(27.8) | 18.1(20.7) |
| Rfree(%)c | 19.7(29.0) | 25.8(32.0) | 26.3(36.2) |
| Mean B-factor (Å2) | 31.7 | 52.1 | 77.2 |
| Wilson B-factor (Å2) | 20.6 | 59.7 | 75.8 |
| r.m.s.d. bond lengths (Å) | 0.006 | 0.007 | 0.010 |
| r.m.s.d. bond angles (°) | 1.18 | 1.39 | 1.39 |
a
Values in parentheses are for highest resolution shells: 1.7-1.74Å, 3.21-3.10Å, and 3.31-3.20Å for murine Igβ monomer, murine Igβ homodimer, and human Igβ homodimer, respectively.
b
Rsym=100×ΣǀIh-<Ih> ǀ/ΣIh, where <Ih> is the mean intensity of multiple measurements of symmetry equivalent reflections.
c
Rfree was calculated using test set of 5%.