Table 2. Viral load using TaqMan RT-PCR in ferrets infected with virus, A/VN/1203/04 (fg, mean±SD).
| Day Postinfection | ||||||
| Group | Organ | 4 | 5 | 6 | 7 | 8 |
| Brain | 8.51×107±1.16×108 a | 6.23×107 b | 3.88×107±5.27×107 | 3.80×107±4.30×107 | ||
| 10 FLD50 | Lung | 2.95×107±1.71×106 | 2.06×104 b | 1.10×106±9.13×105 | 8.51×106±1.20×105 | |
| N.T. | 1.88×104±2.20×104 | 3.40×104±2.28×104 | ||||
| Ileum | 4.44×103±3.32×103 | 485±337 | ||||
| Brain | 3.94×107 b | 7.76×107 b | 3.11×107±4.59×106 | |||
| 1 FLD50 | Lung | 2.70×103 b | 1.09×105 b | 3758±2822 | ||
| N.T. | 9.33×104 b | 5.50×105 b | 8138±1094 | |||
| Ileum | 4.36×103 b | 3.47±1.08 | ||||
Note:
a)
Known concentrations of A/VN/1203/04 viral RNA (serially diluted: 108 to 10 fg) were used as templates and quantitative RT-PCR performed to generate a standard curve. 25∼30 mg of tissue from each organ was used for extraction of viral RNA and dissolved in 50 µl. 3 µl of the vRNA was used as template to perform real-time PCR. Each value represents the average concentration of six reactions based on the standard curve.
b)
indicates value for 1 animal.