FIG 7 .

Effect of the Ffh M domain on the rate of synthesis of LacY-GFP in vivo. E. coli IY228 harboring plasmids encoding arabinose-inducible M and carrying ffs or empty vector were induced with arabinose, and then IPTG was added to induce expression of LacY-GFP. The cells were pulse-labeled with [³⁵S]methionine and [³⁵S]cysteine, and equal samples were withdrawn at the indicated time points. LacY-GFP was immunoprecipitated using anti-GFP antibodies. The precipitates were solubilized in 50 µl SDS sample buffer, and 25 µl was loaded on SDS-PAGE gel (12%), which was then dried and subjected to autoradiography. (B) The amount of labeled LacY-GFP was quantified by densitometry, and the averages of results from three independent assays are shown, with error bars representing standard deviations.