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. Author manuscript; available in PMC: 2010 Aug 13.
Published in final edited form as: Methods Mol Biol. 2009;544:617–636. doi: 10.1007/978-1-59745-483-4_40
Component Amount (μl) Final concentration
TNT Quick Master Mix 40.0
1 mM Methionine 1.0 0.2 mM
T7 TNT PCR enhancer 1.0
5 mM ZnCl2 1.0 0.1 mM
1–2 μg pET15b:ZFN 2.5–5.0 0.02–0.04 μg/μl
dH2O 4.5–2.0