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. Author manuscript; available in PMC: 2010 Aug 13.
Published in final edited form as: J Proteome Res. 2009 Feb;8(2):900–906. doi: 10.1021/pr8005155

Figure 3.

Figure 3

Identification and verification of a lysine-propionylated histone H3 peptide. (A) MS/MS of a tryptic peptide ion (FQKPropSTELLIR) from histone H3 isolated from yeast. (B) MS/MS of a synthetic peptide corresponding to the sequence identified in (A), showing a similar fragmentation pattern. (C) MS/MS of a mixture of the in vivo-derived histone H3 peptide identified in (A) and its synthetic counterpart as indicated in (B). (D) Selected reaction monitoring (SRM) chromatogram of the in vivo-derived histone H3 peptide. (E) SRM chromatogram of the synthetic peptide corresponding to the sequence identified in (A), showing a similar retention time. (F) SRM chromatogram of a mixture of the peptide from the in vivo-derived histone H3 digest and its synthetic counterpart, showing coelution of the two peptides from an HPLC column.