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Total numbers of granule cells are not higher in adrenalectomized (ADX)/clamped rats, and this fact is not due to increased cell death. Granule cell layer (GCL) volume (A), packing density (B), or apoptosis rate (C) were not different in 12-month-old ADX/clamped rats, when compared with age-matched sham-operated (Sham-ADX) rats. D: Examples of TUNEL-positive (upper panel) and pyknotic (lower panel) granule cell nuclei in GCL of a 12-month-old rat. Scale bar = 10 µm.
