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Identification of the genotypes of individual pollen grain. (A) A pollen grain germinated on the agar medium was picked up with a fine pipette tip (marked in the circle). A magnified image of the pollen is lower left. (B) A typical result of genotyping by PCR, showing that seven out of eight pollen grains have wild type OsSUT1 (SUT1+) gene. A PCR product with the DNA template from the leaves of SUT1+/– plant was used as a control.
