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Western blots for C4 enzymes and Rubisco from total soluble proteins extracted from leaves of eight Portulaca species, the C3 species Sesuvium portulacastrum, and the C4 PEP-CK species Spartina anglica. Blots were probed with antibodies raised against PEPC, PPDK, NAD-ME, NADP-ME, PEP-CK, and Rubisco, respectively. Numbers on the left indicate molecular mass in kilodaltons. Subtle differences in blot intensity might occur due to differences in antigenicity between species.
