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. 2010 Jun 30;61(13):3647–3662. doi: 10.1093/jxb/erq178

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Electron microscopy of in situ immunolocalization of glycine decarboxylase (GDC) in chlorenchyma cells of P. oleracea (A, B), P. cryptopetala (D, E), and Sesuvium portulacastrum (G, H), and graphs showing the density of labelling for GDC in BS versus M mitochondria (right panels C, F, and I). (A, D, G) Immunolabelling of GDC in bundle sheath cell mitochondria. (B, E) Lack of immunolabelling for GDC in M cells of two Portulaca species, and (H) showing the presence of gold particles in M mitochondria in S. portulacastrum. (C, F, I) In all graphs the x-axis represents the number of gold particles per μm² of mitochondrial area; for each cell type 10–60 cell fragments were used for counting. The horizontal lines near the base of the bars represent the level of background (number of particles per cell fragment area excluding mitochondria). BS, bundle sheath cell; Ch, chloroplast; M, mesophyll; Mt, mitochondria; PP, phloem parenchyma cell. Scale bars: 0.5 μm.