Fig. 3.

Amino acid sequence of the maize genes encoding arogenate dehydrogenase. (A) Highly conserved region of AroDH-1 and -3 open reading frames showing the Mutator ‘hotspot’. (B) ClustalW amino acid alignment of predicted maize arogenate dehydrogenase proteins. The black shading with white letters indicates residues conserved in all four members, whereas grey shading indicates conservation between two or more of the family members. Solid white line boxes indicate 100% conservation with AroDH genes from other plants, bacteria, and yeast, whereas dashed white boxes indicate close to 100% conservation (Legrand et al., 2006). An asterisk under the conserved histidine residue at position 207 is predicted to be an essential part of the active site (Legrand et al., 2006) and the black arrow at position 223 shows the predicted start of the α-helix-rich, C-terminal dimerization domain (Legrand et al., 2006). The region corresponding to mutator insertion sites indicated in AroDH-1, -2 and -3 is shown with arrows.