Table 1.
Proteins identified by MASCOT with predicted signal peptides
| Annotation/gene familya | SGN unigene | Identified in analysisb | Best hit |
|
| Total ion score | Percentage coverage | |||
| Lipid and putative cuticle related | ||||
| GDSL-motif lipase/hydrolase family protein | SGN-U583101 | A | 37 | 7.5 |
| SGN-U579520 | ABC | 173 | 22 | |
| Inducible plastid lipid-associated protein | SGN-U577010 | C | 76 | 15.9 |
| Lipid transfer protein (LTP) | SGN-U577838 | C | 43 | 11.7 |
| SGN-U579033 | C | 149 | 46.7 | |
| SGN-U579687 | C | 252 | 55.7 | |
| SGN-U580659 | C | 69 | 43.5 | |
| SGN-U581465c | C | 171 | 33.1 | |
| MD-2-related lipid recognition domain-containing (ML) protein | SGN-U577903 | ABC | 93 | 8.6 |
| Defence related | ||||
| Allergen V5/Tpx-1-related family protein | SGN-U578890 | C | 105 | 13.8 |
| Bet v I allergen family protein | SGN-U577856 | AC | 67 | 13.6 |
| Chitinase (GH family 18 and 19)d | SGN-U580366 | BC | 245 | 23.3 |
| SGN-U579068 | C | 244 | 27.3 | |
| SGN-U579551 | C | 219 | 19.8 | |
| SGN-U579696 | C | 72 | 8.7 | |
| SGN-U581507 | C | 91 | 14.4 | |
| Chitin-binding lectin | SGN-U562887 | C | 46 | 7.1 |
| Defensin | SGN-U577872 | BC | 341 | 47.4 |
| SGN-U591780 | C | 62 | 17.5 | |
| Endo β-1,3 glucanase (GH family 17) | SGN-U590837 | C | 102 | 7.8 |
| Hevein-like protein | SGN-U567805 | C | 103 | 12.3 |
| SGN-U579235 | C | 863 | 68.2 | |
| Osmotin-like protein | SGN-U574403 | AC | 473 | 40.7 |
| SGN-U579414 | C | 505 | 31 | |
| SGN-U581103 | C | 574 | 30.6 | |
| Peroxidase | SGN-U581155 | AC | 588 | 21.9 |
| SGN-U583085 | BC | 550 | 23.5 | |
| SGN-U564185 | C | 49 | 7.4 | |
| SGN-U566251 | C | 200 | 25.1 | |
| SGN-U571844 | C | 102 | 8.5 | |
| SGN-U575184 | C | 243 | 36.8 | |
| SGN-U578562 | C | 149 | 6.8 | |
| SGN-U580369 | C | 199 | 17.3 | |
| SGN-U580709 | C | 211 | 34.3 | |
| SGN-U583086 | C | 461 | 23.8 | |
| Peroxiredoxin | SGN-U579538 | C | 74 | 7.4 |
| Polygalacturonase inhibitor protein | SGN-U579059 | AC | 44 | 11.6 |
| PR-1 | SGN-U578279 | C | 180 | 48.7 |
| SGN-U579345 | C | 93 | 12.2 | |
| SGN-U579426 | C | 276 | 34.8 | |
| SGN-U579545 | C | 771 | 52.8 | |
| SGN-U579883 | C | 160 | 25 | |
| Protease | SGN-U578421 | AC | 71 | 6.2 |
| SGN-U582837 | AC | 134 | 8.3 | |
| SGN-U578351 | C | 76 | 4.7 | |
| SGN-U578475 | C | 102 | 7.4 | |
| SGN-U579972 | C | 159 | 6.1 | |
| Protease inhibitor protein | SGN-U573941 | ABC | 194 | 18.3 |
| SGN-U574346 | AC | 83 | 7.3 | |
| SGN-U577283 | C | 194 | 14.8 | |
| SGN-U578389 | C | 163 | 20 | |
| SGN-U578863 | C | 62 | 9.6 | |
| SGN-U585465 | C | 134 | 15.5 | |
| Snakin-like protein | SGN-U578258 | C | 168 | 9 |
| Carbohydrate cell wall metabolism related | ||||
| α-Galactosidase (GH family 27) | SGN-U571081 | C | 102 | 5.9 |
| β-Glucosidase (GH family 1) | SGN-U580766 | A | 49 | 3.9 |
| Expansin | SGN-U577727 | C | 124 | 11.6 |
| Ole e 1 allergen/extensin like | SGN-U563658 | C | 85 | 13.8 |
| Other | ||||
| ADP/ATP translocator like | SGN-U577960 | C | 91 | 5.1 |
| Ascorbate peroxidase | SGN-U578449 | C | 98 | 14 |
| Enolase | SGN-U579393 | C | 581 | 28.9 |
| Formate dehydrogenase | SGN-U579280 | C | 65 | 7.3 |
| Fructokinase | SGN-U586194 | AC | 250 | 9.2 |
| Fructose-bisphosphate aldolase | SGN-U578572 | AC | 209 | 17.6 |
| Glyceraldehyde 3-phosphate dehydrogenase | SGN-U580213 | ABC | 438 | 38.2 |
| Glycine-rich RNA-binding protein | SGN-U578513 | B | 37 | 19.4 |
| Histone H2B | SGN-U579310 | C | 57 | 19.4 |
| Leucine-rich repeat transmembrane protein kinase | SGN-U579197 | C | 122 | 25.2 |
| Malate dehydrogenase | SGN-U565569 | C | 399 | 25.8 |
| Protein disulphide isomerase-like (PDIL) protein | SGN-U575297 | C | 61 | 6.5 |
| SGN-U577569 | C | 108 | 8.8 | |
| Ribulose bisphosphate carboxylase large chain | SGN-U565452 | ABC | 50 | 4.8 |
| SOUL haem-binding protein | SGN-U584870 | A | 48 | 9.2 |
| Strictosidine synthase family protein | SGN-U583542 | AC | 175 | 12.4 |
| Transketolase | SGN-U577918 | C | 109 | 4.2 |
| Unknown | SGN-U593950 | B | 23 | 11.3 |
| SGN-U565851 | C | 41 | 10.7 | |
| SGN-U566943 | C | 52 | 18.2 | |
a
Gene family groupings and annotation based on BLAST search of the NCBI non-redundant database.
b
Analyses [(A) is gel band-based analysis, (B) is gel slab-based analysis, (C) is gel-free analysis] from which members of the protein family were identified. The analysis that yielded the highest protein total ion score is shown in bold.
c
The longest-six frame translation of SGN-U581465, corresponding to CAJ19705, has an incorrect start codon that was manually adjusted before SignalP analysis
d
Glycosyl hydrolase (GH) families, www.cazy.org. The SGN annotation refers to the unigene identifier in the Sol Genomics Network database (www.solgenomics.net).