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. 2010 Aug 13;5(8):e12174. doi: 10.1371/journal.pone.0012174

Figure 1. Platelets do not express LAIR-1 or LAIR-2.

Figure 1

Panel A: Flowcytometric analysis of washed platelets (2×10E5/µl), unstimulated or stimulated for 5 min at RT with 10 µM TRAP or 5000 nM PMA. Upper panels represent staining for CD62L, lower panels represent LAIR-1 staining. Panel B: Western blots containing whole platelet lysates and controls were incubated with antibodies against LAIR-1 (lanes 1–3), LAIR-2 (lanes 4–8) or GpVI (lane 9). Lane 1: purified LAIR-1/Fc; lane 2: purified LAIR-2/Fc; lane 3: whole platelet lysate; lane 4: purified LAIR-1/Fc; lane 5: purified LAIR-2/Fc; lane 6: conditioned medium of non-transfected human 293T cells; lane 7: conditioned medium of human 293T cells secreting LAIR-2; lane 8: whole platelet lysate; lane 9: whole platelet lysate.